8/9/2023 0 Comments Sequence of mrnaIn humans and other complex organisms, mRNA moves from the cell nucleus to the cell cytoplasm (watery interior), where it is used for. This copy, called messenger RNA (mRNA), carries the gene’s protein information encoded in DNA. Interestingly, evidence derived from in situ hybridization and polymerase chain reaction experiments indicate that the mRNA encoding enolase is present in the squid giant axon. Transcription, as related to genomics, is the process of making an RNA copy of a gene’s DNA sequence. Results of Northern analysis revealed a single 1.6 kb mRNA species, the relative abundance of which differs approximately 10-fold between various tissues. The greatest degree of sequence similarity was manifest to the alpha isoform of the human homologue. Cross-species comparison of enolase primary structure reveals that squid enolase shares over 70% sequence identity to vertebrate forms of the enzyme. ![]() ![]() The composite sequence of those cDNA clones is 1575 bp and contains the entire coding region (1302 bp), as well as 66 and 207 bp of 5' and 3' untranslated sequence, respectively. Here we report the isolation of two overlapping cDNA clones and the putative primary structure of the enzyme from the squid (Loligo pealii) nervous system. However, little information is available on the primary sequence of enolase in invertebrates. In mammals, enolase is known to be a dimeric protein composed of distinct but closely related subunits: alpha (non-neuronal), beta (muscle-specific), and gamma (neuron-specific). Enolase is a glycolytic enzyme whose amino acid sequence is highly conserved across a wide range of animal species.
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